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Purification and some properties of pure Cochliobolus lunatus fibrinolytic Enzyme

✍ Scribed by Ahmed F. Abdel-Fattah; Abdel-Mohsen S. Ismail


Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
381 KB
Volume
26
Category
Article
ISSN
0006-3592

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πŸ“œ SIMILAR VOLUMES


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✍ Dr. K. I. Fayek; Sanaa T. El-Sayed πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 404 KB

## Abstract A fibrinolytic enzyme obtained from __B. subtilis__ was purified, using DEAE‐cellulose column chromatography, and gel filtration on Sephadex G‐100. The preparation was homogeneous as tested by gel filtration on Sephadex G‐200, and disc electrophoresis. The molecular weight of this enzy

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## Abstract Two fibrinolytic enzymes isolated from __B. subtilis__ and from __B. polymyxa__ were purified using a five step method. The pH optimum for the enzyme from __B. subtilis__ was 7.2 and for the enzyme from __B. polymyxa__ was 7.0. Both enzymes were activated by Cu^++^. The molecular weigh