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Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115

โœ Scribed by Michael P. Dux; Rick Barent; Jayanta Sinha; Mark Gouthro; Todd Swanson; Ardis Barthuli; Mehmet Inan; John T. Ross; Leonard A. Smith; Theresa J. Smith; Robert Webb; Bonnie Loveless; Ian Henderson; Michael M. Meagher


Book ID
116828221
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
749 KB
Volume
45
Category
Article
ISSN
1046-5928

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An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut + expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(H c )], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes