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Purification and Reconstitution of the Ca2+-Pumping ATPase of Red Blood Cells

✍ Scribed by Paolo Gazzotti; Ernesto Carafoli


Book ID
115638546
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
590 KB
Volume
6
Category
Article
ISSN
1046-2023

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Comparison of the red blood cell Ca2+-AT
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We have compared properties of the red blood cell Ca2(+)-ATPase in two types of preparations: red cell membrane ghosts (enzyme in unfractionated membranes) and after purification (detergent-soluble enzyme). The Ca2(+)-ATPase activity was studied with respect to its requirement for: calmodulin, calci