𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification and properties of the sodium-potassium transport adenosinetriphosphatase from the rectal gland of the spiny dogfish squalus acanthias

✍ Scribed by Hokin, Lowell E.

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
843 KB
Volume
1
Category
Article
ISSN
0091-7419

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The (Na + K)–activated adenosinetriphosphatase (NaK ATPase) has been purified from Lubrol extracts of membranes from the rectal glands of Squalus acanthias. The specific activity of the purified enzyme is 2 to 3 times that previously reported by others after correction of their specific activities for detergent activation. The yield of the enzyme from the membranes is 70%. The enzyme is highly stable both at 0° and in the frozen state. Ninety‐five percent of the enzyme consists of two subunits–the catalytic subunit with a MW of 97,000 and a glycoprotein with a MW of 55,000. At the last stage of purification the enzyme reverts to various membranous forms: the thickness of the membrane is about 80 Å; projections (probably the glycoprotein) of about 40 Å in diameter are seen at regular intervals.

📜 SIMILAR VOLUMES

Glucose transport across ocular barriers
Glucose transport across ocular barriers of the spiny dogfish,Squalus acanthias
✍ Dimattio, Joseph ;Zadunaisky, Joseph A. 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 488 KB 👁 2 views

## Abstract Evidence is presented demonstrating that in the dogfish, __Squalus acanthias__, D‐glucose is transported from the blood to the ocular fluid compartments by a mechanism that is consistent with carrier‐facilitated transport. Across the dogfish aqueous barrier D‐glucose is transported 8.4

A simple procedure for the preparation o
A simple procedure for the preparation of highly purified (sodium + potassium) adenosinetriphosphatase from the rectal salt gland of Squalus acanthias and the electric organ of Electrophorus electrieus
✍ John F. Dixon; Lowell E. Hokin 📂 Article 📅 1978 🏛 Elsevier Science 🌐 English ⚖ 641 KB

A simple purification procedure for the Na,K-ATPase from membranes of the rectal gland of Squalus acanthias or crude microsomal fractions from the electric organ of Hectrophorus electricus is presented here. The purification procedure consists of solubilization of the Na,K-ATPase with the nonionic d

Reproductive endocrinology of female ela
Reproductive endocrinology of female elasmobranchs: Lessons from the little skate (Raja erinacea) and spiny dogfish (Squalus acanthias)
✍ Koob, Thomas J.; Callard, Ian P. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 264 KB 👁 2 views

Conventional classification of reproductive modes in female elasmobranchs fails to account for the diversity in ovarian dynamics that operate during oviparous and viviparous cycles. Delineating this diversity is crucial for understanding the endocrine regulation of the manifold physiological mechani

Purification and molecular properties of
Purification and molecular properties of the (sodium + potassium)-adenosinetriphosphatase and reconstitution of coupled sodium and potassium transport in phospholipid vesicles containing purified enzyme
✍ Hokin, Lowell E. 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 792 KB

## Abstract Recent work in our laboratory on the purification and characterization of the (sodium + potassium)‐activated adenosinetriphosphatase (NaK ATPase) has been reviewed. Two enzymes have been purified, that from the rectal salt gland of the spiny dogfish, __Squalus acanthias__ and that from