Purification and fluorometric assay of p
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Hideyoshi Yokosawa; Hiroshi Ito; Shigeki Murata; Shin-ichi Ishii
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Article
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1983
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Elsevier Science
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English
β 461 KB
Proteinase A was purified from commercial baker's yeast to homogeneity by using affinity chromatography. Simple and sensitive fluorometric assay procedures were developed for this enzyme, where Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide and dimethylcasein were used as synthetic sub