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Purification and properties of phosphoenolpyruvate carboxylase from the germinating peanut cotyledon

✍ Scribed by Hitoshi Maruyama; M.Daniel Lane

Book ID
115791491
Publisher
Elsevier Science
Year
1962
Weight
662 KB
Volume
65
Category
Article
ISSN
0006-3002

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Purification and molecular and kinetic p
Purification and molecular and kinetic properties of phosphoenolpyruvate carboxylase fromAmaranthus viridisL. leaves
✍ A. A. Iglesias; D. H. GonzΓ‘lez; C. S. Andreo πŸ“‚ Article πŸ“… 1986 πŸ› Springer-Verlag 🌐 English βš– 772 KB

Phosphoenolpyruvate carboxylase (EC 4.1.1.31) was purified 43-fold from Amaranthus viridis leaves by using a combination of ammonium-sulphate fractionation, chromatography on O-(diethylaminoethyl)-cellulose and hydroxylapatite, and filtration through Sepharose 6B. The purified enzyme had a specific