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Purification and properties of mouse thymus thymidylate synthase. comparison of the enzyme from mammalian normal and tumour tissues

✍ Scribed by Wojciech Rode; Joanna Cieśla; Zbigniew Zieliński; Barbara Kedzierska

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
669 KB
Volume
18
Category
Article
ISSN
0020-711X

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✍ Chung-Her Jenh; Lakshmi G. Rao; Lee F. Johnson 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 671 KB

Thymidylate synthase (TS) activity is very low in resting mouse 3T6 fibroblasts but increases sharply in growth-stimulated cells at about the same time the cells enter S phase. To study the mechanism responsible for the increase in TS level, we isolated a 5-fluorodeoxyuridine (5-FdUrd)-resistant cel