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Purification and properties of monomeric (G1) forms of acetylcholinesterase secreted by Nippostrongylus brasiliensis

✍ Scribed by Michael E Grigg; Liang Tang; Ayman S Hussein; Murray E Selkirk

Book ID
114146407
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
230 KB
Volume
90
Category
Article
ISSN
0166-6851

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## Abstract The mutant M301A of the acetylcholinesterase B from __Nippostrongylus brasiliensis__ (NbAChE) was produced in a high‐cell‐density fermentation of a recombinant methylotrophic yeast __Pichia pastoris__. Dissolved oxygen (DO) spikes were used as an indicator for feeding the carbon source.