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Purification and properties of methanol dehydrogenase from Methylophaga marina

โœ Scribed by Janvier, Monique; Gasser, Francis

Book ID
122475013
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
638 KB
Volume
69
Category
Article
ISSN
0300-9084

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Methanol dehydrogenase (MDH) from Methylocystis sp. GB 25, which belongs to the group I1 of methanotrophic bacteria, is able to catalyse the oxidation of methanol to formate directly. The enzyme was purified 20-fold by a 5 step procedure to electrophoretic homogeneity. After cell disruption by Frenc