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Purification and properties of l(−)-carnitine dehydrogenase from Agrobacterium sp.

✍ Scribed by Henning Hanschmann; Ralf Ehricht; Hans-Peter Kleber

Book ID
116126990
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
714 KB
Volume
1290
Category
Article
ISSN
0304-4165

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✍ Dr. Mitsugi Iida; Shin'Ichi Ohtsuki; Shigeru Mineki 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

Formate: NAD+ oxidoreductase (EC 1.2.1.2) with high affinity to formate was purified from a facultative formate-utilizing Agrobacterium sp. MIL 1052. From a cell extract, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, Sephadex (3-2