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Purification and Properties of Insulin Receptor Ectodomain from Large-Scale Mammalian Cell Culture

✍ Scribed by Leah Cosgrove; George O. Lovrecz; Amanda Verkuylen; Lilliana Cavaleri; Loyce A. Black; John D. Bentley; Geoffrey J. Howlett; Peter P. Gray; Colin W. Ward; Neil M. McKern


Book ID
115644881
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
181 KB
Volume
6
Category
Article
ISSN
1046-5928

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## Abstract Transient expression of recombinant proteins in mammalian cell culture in a 100‐L scale requires a large quantity of plasmid that is very labour intensive to achieve with shake flask cultures and commercially available plasmid purification kits. In this paper we describe a process for p