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Purification and properties of an endo-β-1,4-glucanase from strawberry and down-regulation of the corresponding gene,cel1

✍ Scribed by Lindsey C. Woolley; David J. James; Kenneth Manning

Publisher
Springer-Verlag
Year
2001
Tongue
English
Weight
263 KB
Volume
214
Category
Article
ISSN
0032-0935

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✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular