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Purification and properties of an acetyl specific carboxylesterase fromNocardia mediterranei

✍ Scribed by Hans-Peter Schär; Daniel Gygax; Gerardo M. Ramos Tombo; Oreste Ghisalba

Publisher
Springer
Year
1988
Tongue
English
Weight
472 KB
Volume
27
Category
Article
ISSN
1432-0614

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Purification and properties of an NADP+-specific isocitrate dehydrogenase fromRhizobium meliloti
✍ P. T. Chandrasekharan Nambiar; Y. I. Shethna 📂 Article 📅 1976 🏛 Springer Netherlands 🌐 English ⚖ 511 KB

An NADP+-specific isocitrate dehydrogenase has been purified and characterized from Rhizobium meliloti. The enzyme showed Mn++ or Mg++ requirement. The apparent Km values were 2.00 x 10(-5) M and 1.51 x 10(-5) M for DL-isocitrate and NADP+, respectively. The enzyme was inhibited by ATP, to a lesser