Purification and properties of a glycopr
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M. Carmen López; Dr. Angel Domínguez
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Article
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1988
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John Wiley and Sons
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English
⚖ 904 KB
An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz