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Purification and preliminary crystallographic studies on the sporulation response regulatory phosphotransferase protein, spo0B, from Bacillus subtilis

✍ Scribed by Xiao Zhen Zhou; J.M. Whiteley; J.A. Hoch; K.I. Varughese

Book ID: 101228522
Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 133 KB
Volume: 27
Category: Article
ISSN: 0887-3585
DOI: 10.1002/(sici)1097-0134(199704)27:4<597::aid-prot11>3.0.co;2-f

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✦ Synopsis

The phosphotransferase protein Spo0B, a component of the sporulation signal transduction system in Bacillus subtilis was expressed from the Escherichia coli strain BL21DE3. It was purified, crystallized, and 2.25 A data measured using the synchrotron source at the Stanford Linear Accelerator Center. The search for heavy atom derivatives is in progress.