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Purification and preliminary characterization of an extracellular pullulanase fromThermoanaerobiumTok6-B1

✍ Scribed by Adrian R. Plant; Robyn M. Clemens; Roy M. Daniel; Hugh W. Morgan

Publisher
Springer
Year
1987
Tongue
English
Weight
589 KB
Volume
26
Category
Article
ISSN
1432-0614

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## Abstract β‐1.3‐1.4‐glucanase (E.C.3.2.1.73) was obtained in highly purified form from the culture fluid of __Bacillus__ IMET B 376 by precipitation with ammonium sulfate, adsorption on CM‐cellulose and then affinity chromatography on lichenan‐Sepharose 4B. The purified enzyme was active on liche