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Purification and partial characterization of the alkaline phosphatase from Allolobophora caliginosa

โœ Scribed by Giovannini, Elvio; Principato, Giovanni B.; Ambrosini, Maria V.; Menghini, Annarita; Dell'agata, Massimo


Book ID
121966393
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
227 KB
Volume
61
Category
Article
ISSN
1096-4959

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Purification and partial characterizatio
โœ Per ร…ke Holmgren; Torgny Stigbrand; Gunhild Beckman ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 461 KB

The I variant of placental alkaline phosphatase was purified to homogeneity by means of DEAE-cellulose chromatography, isoelectric focusing, and gel filtration on AcA-34. The specific activity of the I variant was found to be 3.33 micronkat/mg. The enzyme is a dimer with an isoelectric point of 4.6