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Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver

โœ Scribed by Patrick Y.-K. Wong

Book ID
113124419
Publisher
Elsevier Science
Year
1981
Weight
525 KB
Volume
659
Category
Article
ISSN
0005-2744

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Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w