Purification and partial characterization of cell surface glycoprotein from extremely halophilic archaeonHaloarcula japonicastrain TR-1

✍ Scribed by Satoshi Nakamura; Shin Mizutani; Hiromichi Wakai; Hiroshi Kawasaki; Rikizo Aono; Koki Horikoshi

• Book ID: 104633936
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 275 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00130354

✦ Synopsis

Haloarculajaponica has a glycoprotein on its cell surface. The cell surface glycoprotein of H. juponica was purified and characterized. It had an apparent molecular mass of 180 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The carbohydrate content was about 12% (wt/wt). The polypeptide portion contained a large proportion ot acidic amino acids, and the sequence of 18 N-terminal amino acids was determined.