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Purification and partial characterization of a cohaemolysin (CAMP-factor) produced by Streptococcus canis

✍ Scribed by Kemal Gürtük; Christoph Lämmler


Book ID
109315131
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
267 KB
Volume
64
Category
Article
ISSN
0378-1097

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Rapid purification and partial character
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Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands