Affinity purification of renal dipeptida
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Michael J.M. Hitchcock; Cathy A. Farrell; Susan Huybensz; Bing-Yuh Luh; David J.
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Article
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1987
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Elsevier Science
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English
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A new method is described for purification of the dehydropeptidase I enzyme, renal dipeptidase (EC 3.4.13. I I). The six steps used are homogenization ofthe tissue, extraction with Triton X-100, sedimentation of insoluble material, and ion-exchange, size-exclusion, and affinity chromatographies. The