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Purification and isotype analysis of protein kinase C from rat liver nuclei

✍ Scribed by Mariëlle P de Moel; Sjenet E Van Emst-De Vries; Peter H.G.M Willems; Jan Joep H.H.M De Pont


Book ID
117756396
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
395 KB
Volume
30
Category
Article
ISSN
1357-2725

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✍ Yi Qu; Joseph Torchia; Than Duc Phan; Amar Kumar Sen 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 608 KB

A calcium-sensitive, phospholipid-dependent protein kinase (protein kinase C) and its three isozymes were purified from rat heart cytosolic fractions utilizing a rapid purification method. The purified protein kinase C enzyme showed a single polypeptide band of 80 KDa on SDS-polyacrylamide gel elect