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Purification and Electron Microscopic Characterization of the Membrane Subunit (IICBGlc) of the Escherichia coli Glucose Transporter

✍ Scribed by Jianping Zhuang; Regula Gutknecht; Karin Flükiger; Lorenz Hasler; Bernhard Erni; Andreas Engel


Book ID
115567870
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
446 KB
Volume
372
Category
Article
ISSN
0003-9861

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Partial purification of active delta and
✍ Smith, Jeffrey B. ;Sternweis, Paul C. ;Heppel, Leon A. 📂 Article 📅 1975 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 393 KB

We have partially purified active delta and epsilon subunits of the E. coli membranebound MgZf-ATPase (ECF1). Treating purified ECFl with 50% pyridine precipitates the major subunits (a, 0, and y ) of the enzyme, but the two minor subunits (6 and E ) , which are present in relatively small amounts,