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Purification and characterization of β-mannanase from Bacillus licheniformis for industrial use

✍ Scribed by Jun Zhang; Zhimin He; Kun Hu

Book ID: 110226762
Publisher: Springer Netherlands
Year: 2000
Tongue: English
Weight: 47 KB
Volume: 22
Category: Article
ISSN: 0141-5492
DOI: 10.1023/a:1005644414762

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## Abstract β‐1.3‐1.4‐glucanase (E.C.3.2.1.73) was obtained in highly purified form from the culture fluid of __Bacillus__ IMET B 376 by precipitation with ammonium sulfate, adsorption on CM‐cellulose and then affinity chromatography on lichenan‐Sepharose 4B. The purified enzyme was active on liche