Purification and characterization of two thermostable protease fractions from Bacillus megaterium
โ Scribed by Asker, Mohsen M.S.; Mahmoud, Manal G.; El Shebwy, Khalid; Abd el Aziz, Mohamed S.
- Book ID
- 121801117
- Publisher
- Springer (Biomed Central Ltd.)
- Year
- 2013
- Tongue
- English
- Weight
- 786 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1687-157X
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## Abstract An alkaline serine protease produced by __Bacillus megaterium__ RRM2 isolated from the red alga, __Kappaphycus alvarezii__ (Doty) Doty ex Silva was studied for the first time and the same analyzed for the production of protease in the present study. Identification of the bacterium was d
## Abstract An extracellular lipase isolated from __Bacillus megaterium__ AKGโ1 had an optimum activity at 55 ยฐC/pH 7.0. It retained 100% activity at 50 ยฐC for 30 min with a half life of 30 min at 70 ยฐC. A 20โ70% increase in lipase activity was observed in presence of acetone (20% v/v), DMSO (20% v