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Purification and characterization of two forms of DNA polymerase .alpha. from HeLa cell nuclei

โœ Scribed by Enomoto, Takemi; Tanuma, Seiichi; Yamada, Masaatsu

Book ID
127236406
Publisher
American Chemical Society
Year
1983
Tongue
English
Weight
556 KB
Volume
22
Category
Article
ISSN
0006-2960

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The major pol alpha activity of CHO cells was purified 2 800-fold to near homogeneity and was characterized with respect to its physical and catalytic properties. The purified enzyme, upon analysis in denaturing 'activity' gels, displayed a major, 120 kilodalton, catalytically active core and two mi