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Purification and characterization of three neutral extracellular isoperoxidases from rye leaves

✍ Scribed by Satoshi Murakami; Hidenari Takahara; Masakazu Shiraiwa

Book ID
108242543
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
400 KB
Volume
68
Category
Article
ISSN
0031-9422

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Homogeneous phosphoribulokinase (PRK ; ATP : D-ribulose-5-phosphate 1 -phosphotransferase, EC 2.7.1.19) was isolated from wheat leaves with a specific activity of 15 pkat mg-' protein. The purification included ammonium sulfate cuts, isoelectric precipitation, and hydrophobic and affinity chromatogr