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Purification and Characterization of the Recombinant Form of Acyl CoA Oxidase 3 from the Yeast Yarrowia lipolytica

โœ Scribed by Yi-Shan Luo; Hui-Jie Wang; K.V Gopalan; D.K Srivastava; Jean-Marc Nicaud; Thierry Chardot


Book ID
115568481
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
140 KB
Volume
384
Category
Article
ISSN
0003-9861

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โœ M. Carmen Lรณpez; Dr. Angel Domรญnguez ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 904 KB

An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz