Purification and characterization of the extracellular and cell-bound lipases from aPenicillium cyclopiumvariety

✍ Scribed by Danièle Druet; Najia Abbadi; Louis-Claude Comeau

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 404 KB
• Volume: 37
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00174840

✦ Synopsis

A new strain of Penicilfium cyclopium was f o u n d on m o u l d y G r e n o b l e walnuts. F r o m the culture filtrate, two new extracellular lipases (A-and B-lipase) were isolated and characterized. The mycelium contained one o f the two forms of lipase (B-lipase). The specificity of the cell-bound lipase linked to the mycelium was then studied. The enzyme was specific to shortchain h o m o g e n e o u s triacylglycerols; it preferred to attack the o-position rather t h a n the p -p o s i t i o n , and showed stereospecificity on the sn-1 position.