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Purification and characterization of proteinase yscJ, a new yeast peptidase

✍ Scribed by Jean-Claude WAGNER; Dieter H. WOLF


Book ID
115129055
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
588 KB
Volume
203
Category
Article
ISSN
1432-1327

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Purification and fluorometric assay of p
✍ Hideyoshi Yokosawa; Hiroshi Ito; Shigeki Murata; Shin-ichi Ishii πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 461 KB

Proteinase A was purified from commercial baker's yeast to homogeneity by using affinity chromatography. Simple and sensitive fluorometric assay procedures were developed for this enzyme, where Suc-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide and dimethylcasein were used as synthetic sub