The reduced methylviolegen-nitrate reductase of the obligate thermophile, Bacillus stearothermophilus NCA strain 2 I84 (ATCC 120 16), has been purified to electrophoretic homogeneity, 53-fold with a yield of 12.5%. The purification procedure involved solubilization with octyl glucoside, ammonium sul
Purification and Characterization of Nitrate Reductase from the Halotolerant CyanobacteriumAphanothece halophytica
โ Scribed by Soraya Thaivanich; Aran Incharoensakdi
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 213 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1573-0972
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Nitrite reductase (NiR) isolated from barley (Hordeum vulgare L.) roots was stabilized in a buffer solution containing a sulfhydryl-reducing reagent and glycerol. The enzyme was purified 340fold by ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50, Sephadex G-200 and DEAE-cellu