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Purification and characterization of NADP+-linked isocitrate dehydrogenase from an alkalophilic Bacillus

โœ Scribed by Shitsuw Shikata; Katsuya Ozaki; Shuji Kawai; Susumu Ito; Kikuhiko Okamoto


Book ID
113266898
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
589 KB
Volume
952
Category
Article
ISSN
0167-4838

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Purification and properties of an NADP+-
โœ P. T. Chandrasekharan Nambiar; Y. I. Shethna ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 511 KB

An NADP+-specific isocitrate dehydrogenase has been purified and characterized from Rhizobium meliloti. The enzyme showed Mn++ or Mg++ requirement. The apparent Km values were 2.00 x 10(-5) M and 1.51 x 10(-5) M for DL-isocitrate and NADP+, respectively. The enzyme was inhibited by ATP, to a lesser