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Purification and characterization of myosin from wheat mitochondria

✍ Scribed by Heping Zhao; Aixiao Liu; Guoqin Liu; Lungfei Yen

Book ID
111783455
Publisher
Springer
Year
2002
Tongue
English
Weight
529 KB
Volume
47
Category
Article
ISSN
1001-6538

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Homogeneous phosphoribulokinase (PRK ; ATP : D-ribulose-5-phosphate 1 -phosphotransferase, EC 2.7.1.19) was isolated from wheat leaves with a specific activity of 15 pkat mg-' protein. The purification included ammonium sulfate cuts, isoelectric precipitation, and hydrophobic and affinity chromatogr