Purification and characterization of immunosuppressive factor derived from human trophoblast and malignant cells
โ Scribed by Kiichiro Itoh; Noboru Matsuzaki; Yutaka Izumi; Hiromi Fujiwara; Toshiyuki Hamaoka
- Book ID
- 119090209
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 128 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0165-0378
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Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000
## An immunoglobulin (Ig) production stimulating factor (IPSF) for hybridomas was found in spent medium of the human B lymphoblastoid cell line, HO-323. The IPSF was purified by serial use of DEAE chromatography, ultrafilnation, gel filtration and HPLC-DEAE chromatography. Purified IPSF was estim