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Purification and characterization of human fibroblast-derived hybridoma growth factor identical to T-cell-derived B-cell stimulatory factor-2 (interleukin-6)

✍ Scribed by Jo VAN DAMME; Sylvie CAYPHAS; Jacques VAN SNICK; René CONINGS; Willy PUT; Jean-Pierre LENAERTS; Richard J. SIMPSON; Alfons BILLIAU


Book ID
115125301
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
908 KB
Volume
168
Category
Article
ISSN
1432-1327

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Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000