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Purification and Characterization of Dihydropyrimidine Dehydrogenase fromAlcaligenes eutrophus

✍ Scribed by Ulrich Schmitt; Karin Jahnke; Katrin Rosenbaum; Paul F. Cook; Klaus D. Schnackerz


Book ID
115566161
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
135 KB
Volume
332
Category
Article
ISSN
0003-9861

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A NAD-linked L(+)-lactate dehydrogenase (EC 1.1.1.27) was isolated from Alcaligenes eutrophus N9A. During purification advantage was taken of the high affinity of Matrex TM Gel Green A for this enzyme in crude extracts. One ml of this medium adsorbed 2660 U lactate dehydrogenase if 129 ml crude extr