Purification and characterization of defensins from cystic fibrosis sputum
✍ Scribed by L. B. Soong; T. Ganz; A. Ellison; G. H. Caughey
- Publisher
- SP Birkhäuser Verlag Basel
- Year
- 1997
- Tongue
- English
- Weight
- 117 KB
- Volume
- 46
- Category
- Article
- ISSN
- 1420-908X
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Sputum analysis is a useful technique for the study of airway inflammation. In asthma, dithiothreitol (DTT) is used to disperse cells from surrounding mucus; however, the applicability of these processing methods to cystic fibrosis (CF) sputum is unknown. In order to compare two methods for processi
## Abstract Disorders of the respiratory system, such as cystic fibrosis (CF), involve the infiltration and activation of airway inflammatory cells, including neutrophils. This leads to the secretion of peroxidases, which react further with substrates in solution to produce oxidative metabolites, s