Purification and characterization of an α-glucosidase from the hepatopancreas of the shrimp Penaeus japonicus (Crustacea: decapoda)
✍ Scribed by Nin-Nin Chuang; Kung-Shih Lin; Bei-Chia Yang
- Book ID
- 113324056
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 537 KB
- Volume
- 102
- Category
- Article
- ISSN
- 1096-4959
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Penaeus vannamei is an omnivorous species, and it can be assumed that a high level of carbohydrates is necessary for growth. Alpha-glucosidases are important enzymes necessary for the ultimate liberation of glucose residues from various carbohydrates. Using acarbose affinity chromatography, a glycos
## Abstract An acidic β‐galactosidase was purified approximately 2,500‐fold to homogeneity from the shrimp __Penaeus japonicus__. with a final specific activity of 4,000 units/mg of protein. SDS‐polyacrylamide gel electrophoresis revealed the monomers of the acidic β‐galactosidase to have a relativ
## Abstract Phosphotyrosyl protein phosphatase, purified from the hepatopancreas of __Panaeus japonicus__, is a monomeric enzyme with a relative mass (Mr) of 28,000, as estimated by size‐exclusion FPLC on a Superose^TM^ 12. It has a hydrophobic domain and can be extracted with the detergent CHAPS.