Purification and Characterization of an NADPH-Cytochrome P450 (Cytochrome c) Reductase from Spearmint (Mentha spicata) Glandular Trichomes
β Scribed by Krishan Ponnamperuma; Rodney Croteau
- Book ID
- 115566020
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 154 KB
- Volume
- 329
- Category
- Article
- ISSN
- 0003-9861
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π SIMILAR VOLUMES
NADPH-cytochrome P-450 oxidoreductase (P-450 red) transfers reducing equivalents from NADPH to cytochrome P-450 (P-450) in the monooxygenase system. Detergent solubilized proteins from the membrane fraction of neonatal rat epidermis were purified by 2',5'-ADP-agarose affinity column chromatography.
NADPH:cytochrome c (cytochrome P-450) reductase (Fp) from hamster liver microsomes has been purified to near homogeneity using a simple and rapid method. Microsomes were treated with the detergent Chaps (3-[(3-cholamidopropyl)dimethylammonio]propanesulfonic acid) in combination with 0.07% protamine