Purification and characterization of an NADH oxidase fromEubacterium ramulus
โ Scribed by Claudia Herles; Annett Braune; Michael Blaut
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 45 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0302-8933
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๐ SIMILAR VOLUMES
\(\alpha\)-NADH can be determined in the presence of high concentrations of \(\beta\)-NADH using anion exchange HPLC combined with oxidation of \(\beta\)-NADH by lactate dehydrogenase. The method is suited for the detection of a large number of impurities. Residual absorption is a poor measure of \(
The anion-exchange chromatography of commercial NADH using a potassium bicarbonate solution as eluent yields highly pure NADH with good stability\_ Twelve compounds are also separated which act as dehydrogenase inhibitors. The main impurities are further characterized. The compound mainly responsibi