✦ LIBER ✦

Purification and characterization of agar from digenea simplex

✍ Scribed by Mohamed M. El-Sayed

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 542 KB
Volume: 118
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(83)88040-9

No coin nor oath required. For personal study only.

✦ Synopsis

The agar of Digenea simplex was isolated in 26.12% yield. The agarose fraction (19.59%) was purified by anion-exchange chromatography on DEAE-Sephadex A50. Acid hydrolysis of the agar isolated gave galactose, 3,6-anhydrogalactose, 6-0-methylgalactose, glucose, and xylose. Acid hydrolysis of the agarose fraction afforded galactose, 3,6_anhydrogalactose, and 6-O-methylgalactose. The content of SO,"-was lowered from 2.33 to 0.07% by fractionation.

The molecular weight and rheological properties of the agarose isolated were studied.

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