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Purification and characterization of adenosine triphosphatase from eel liver mitochondria

✍ Scribed by Alessandra Baracca; Mauro Degli Esposti; Giovanna Parenti Castelli; Giancarlo Solaini

Book ID
113323974
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
527 KB
Volume
101
Category
Article
ISSN
1096-4959

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Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute o