Purification and characterization of a xylanase and an arabinofuranosidase from Bacillus polymyxa
✍ Scribed by Pilar Morales; Alejo Madarro; Agustí Flors; JoséM. Sendra; JoséA. Pérez-González
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 735 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-0229
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A white-rot fungus Dichomitus squalens, when grown on 1% wheat-straw glucuronoarabinoxylan under aerated submerged conditions, secreted an a-~arabinofuranosidase (4.3 nkat/mL). The enzyme was purified 70-fold by ammonium sulfate precipitation, chromatofocusing on PBE 94, gel filtration on Ultrogel A
## Abstract β‐1.3‐1.4‐glucanase (E.C.3.2.1.73) was obtained in highly purified form from the culture fluid of __Bacillus__ IMET B 376 by precipitation with ammonium sulfate, adsorption on CM‐cellulose and then affinity chromatography on lichenan‐Sepharose 4B. The purified enzyme was active on liche