Purification and characterization of a serine proteinase inhibitor from human articular cartilage
β Scribed by BURKHARDT, H; KASTEN, M; SILKE, R
- Book ID
- 122547716
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 511 KB
- Volume
- 924
- Category
- Article
- ISSN
- 0304-4165
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The major low molecular weight serine proteinase inhibitor of human articular cartilage was purified to homogeneity as determined by single-peak elution with 4 high resolution techniques. The purified protein was found to be a potent inhibitor of human leukocyte elastase and cathepsin G, as well as
## Abstract Human alpha one proteinase inhibitor (Ξ±1βPI) was cloned and expressed in __Aspergillus niger__, filamentious fungus that can grow in defined media and can perform glycosylation. Submerged culture conditions were established using starch as carbon source, 30% dissolved oxygen concentrati