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Purification and characterization of a ribonuclease specific for poly(U) and poly(C) from the larvae of Ceratitis capitata

✍ Scribed by Diamantis C. SIDERIS; Emmanuel G. FRAGOULIS


Book ID
115125042
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
635 KB
Volume
164
Category
Article
ISSN
1432-1327

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A specific alkaline phosphatase (ALPase) from t h e integument of white pupae has been purified 500-fold. The purification procedure included solubilization with Triton X-100, butanol extraction, fractionation with ammonium sulfate, and chromatography o n concanavalin A-Sepharose, Sephadex G-200, an