Purification and Characterization of a Recombinant G-Protein-Coupled Receptor, Saccharomyces cerevisiae Ste2p, Transiently Expressed in HEK293 EBNA1 Cells †
✍ Scribed by Shi, Chunhua; Shin, Youn-Ok; Hanson, John; Cass, Brian; Loewen, Michele C.; Durocher, Yves
- Book ID
- 121078843
- Publisher
- American Chemical Society
- Year
- 2005
- Tongue
- English
- Weight
- 345 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0006-2960
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We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us
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