Purification and characterization of a peanut-agglutinin-binding pancreatic-cancer-related serum mucus glycoprotein
✍ Scribed by C. K. Ching; Jonathan M. Rhodes
- Book ID
- 102865330
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- French
- Weight
- 729 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
A high-molecular-weight (approximately 3.5 X lo' kDa) peanut agglutinin (PNAkbinding glycoprotein was previously identified as a tumour marker in pancreatic cancer sera. In the present study the density of this glycoprotein has been estimated and further analysis performed using sequential lectin blotting combined with serial mild acid hydrolysis followed by repeated Smith degradation. This has allowed partial in-situ characterization of the carbohydrate side-chains. The PNA-binding glycoprotein variably expresses the blood group H and sialylated Lewis' antigens. H and sialyl Lea expression disappeared after mild acid hydrolysis and T (PNA acceptor) disappeared after the first Smith degradation step.