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Purification and characterization of a new recombinant factor VIII (N8)

✍ Scribed by L. THIM; B. VANDAHL; J. KARLSSON; N. K. KLAUSEN; J. PEDERSEN; T. N. KROGH; M. KJALKE; J. M. PETERSEN; L. B. JOHNSEN; G. BOLT; P. L. NØRBY; T. D. STEENSTRUP


Book ID
108775377
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
741 KB
Volume
16
Category
Article
ISSN
1351-8216

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Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands