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Purification and characterization of 20 S proteasome from wheat leaves

✍ Scribed by Pedro Fernández Murray; Carla V. Giordano; Susana Passeron; Atilio J. Barneix

Book ID
117383473
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
939 KB
Volume
125
Category
Article
ISSN
0168-9452

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Homogeneous phosphoribulokinase (PRK ; ATP : D-ribulose-5-phosphate 1 -phosphotransferase, EC 2.7.1.19) was isolated from wheat leaves with a specific activity of 15 pkat mg-' protein. The purification included ammonium sulfate cuts, isoelectric precipitation, and hydrophobic and affinity chromatogr