Purification and characterisation of two extremely halotolerant xylanases from a novel halophilic bacterium
โ Scribed by PeterLangborg Wejse; Kjeld Ingvorsen; KimKusk Mortensen
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 327 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1431-0651
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Halophilic and halotolerant bacteria were isolated from soil samples of a Japanese salt field, an environment where salt concentrations vary annually. From 1 g of each of the five samples collected, over 1 ร 103 bacterial colonies (colony forming units (cfu)g-l) grew on agar medium containing 2M Na
A major xylanase from Fusarium oxysporum was purified to homogeneity by gel filtration, affinity, and ion-exchange chromatographies. It has a molecular mass of 60.2 kDa and pI of 6.6 and was optimally active at pH 7.4 and at 50 degrees C. The enzyme was stable over the pH range 5.8-8.2 at 40 degrees